WesternBright Quantum HRP Substrate K-12042-D10 from Advansta

Product Specs
ItemWesternBright Quantum HRP Substrate
CompanyAdvansta
Price Pricing Info Supplier Page
Catalog NumberK-12042-D10
Quantity100ml
ApplicationsWestern blotting, ELISA
Brand/TrademarkAdvansta

Specifications/Features

WesternBright® Quantum® is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples. Features- Sensitive - detect attomoles of protein per band. Quantitative - linear range of signal with respect to protein amount exceeds 3 orders of magnitude. Low background - for high signal to noise. Long lasting signal - image blots hours after substrate incubation. Versatile - Optimized for CCD imaging, and compatible with film detection

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociationNature Structural & Molecular BiologyNovember 1, 2023Jinglong Wang, Julien Le Gall, Richard L. Frock, Terence R. Strickanti-β-actin, 1:4,000, SC-47778, Santa Cruz Biotechnology) and horseradish peroxidase (HRP) substrate (K-12042-D10, Advansta) were used for the western blotting experiments. GFP knock-out and detection by flow cytometry The

(2) Identification of Molecular Mechanisms Responsible for the MMP-9-1562C/T Dependent Differential Regulation of Matrix Metalloproteinase-9 Expression in Human Neuron-like CellsGenes (Basel)October 31, 2023Sylwia Pabian-Jewuła, Magdalena Ambrożek-Latecka, Aneta Brągiel-Pieczonka, Klaudia Nowicka, Marcin Rylski4 °C. Proteins were detected by Western Bright Quantum HRP Substrate (Advansta, San Jose, CA, USA, #K-12042-D10), and chemiluminescence was measured using an Alliance apparatus (UVITEC, Cambridge, UK). 2.7. Gel Shift Assay

(3) Molecular fingerprints in the hippocampus of alcohol seeking during withdrawalResearch SquareSeptember 29, 2023Roberto Radwańska, Roberto Salamian, Ahmad Skonieczna, Bartosz Skonieczna, Bartosz Gielniewski, Bartek Gielniewski, Zofia Harda, Robbert Havekes, Robbert Havekes, Kasia Radwanskaagain. The membranes were visualized by G-Box apparatus using chemiluminescent reagent (Advansta, #K-12042-D10). TGX stain-free gels from Bio-Rad (#4568095) were used to standardize protein loading on gels [79]. Statistical

(4) Fluid shear stress-modulated chromatin accessibility reveals the mechano-dependency of endothelial SMAD1/5-mediated gene transcriptioniScienceSeptember 15, 2023Jerome Jatzlau, Paul-Lennard Mendez, Aybuge Altay, Lion Raaz, Yufei Zhang, Sophia Mähr, Akin Sesver, Maria Reichenbach, Stefan Mundlos, Martin Vingron, et al.assays ,, QuickExtract™ DNA Extraction Solution,Lucigen,Cat#QE09050 WesternBright Quantum kit,Advansta,Cat#K-12042-D10 NucleoSpin RNA XS isolation kit,Macherey-Nagel,Cat#740902.50 MinElute Reaction Cleanup Kit,Qiagen,Cat#28206 Tagment

(5) Molecular fingerprints in the hippocampus of alcohol seeking during withdrawalbioRxivSeptember 1, 2023Roberto Pagano, Ahmad Salamian, Edyta Skonieczna, Bartosz Wojtas, Bartek Gielniewski, Zofia Harda, Anna Cały, Robbert Havekes, Ted Abel, Kasia Radwanskaagain. The membranes were visualized by G-Box apparatus using chemiluminescent reagent (Advansta, #K-12042-D10). TGX stain-free gels from Bio-Rad (#4568095) were used to standardize protein loading on gels 79. Statistical

(6) The CSB chromatin remodeler regulates PARP1- and PARP2-mediated single-strand break repair at actively transcribed DNA regionsNucleic acids researchAugust 11, 2023Bilkis R, Lake RJ, Cooper KL, Tomkinson A, Fan HY.developed using either SuperSignal West Pico (ThermoFisher) or WesternBright Quantum (Advansta, cat. no. K-12042-D10, San Jose, CA, USA) HRP chemiluminescent substrates, exposed to X-ray film, and imaged with a Konica

(7) TP53BP1, a dual-coding gene, uses promoter switching and translational reinitiation to express a smORF proteiniScienceMay 19, 2023Marta A. Inchingolo, Aurélie Diman, Maxime Adamczewski, Tom Humphreys, Pascale Jaquier-Gubler, Joseph A. Curranextracts,Promega,#L4380 Dual-Luciferase Reporter Assay System,Promega,#E1910 WesternBright™ Quantum,Advansta,#K-12042-D10 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293T,ATCC,CRL-3216 HeLa,ATCC,CCL-2 MCF-7,ATCC,HTB-22 MCF-10,ATCC,CRL-10317 THP-1

(8) Analysis of a rare progeria variant of Barrier-to-autointegration factor in Drosophila connects centromere function to tissue homeostasisCellular and Molecular Life SciencesMarch 1, 2023Tingting Duan, Srikantha Thyagarajan, Anastasia Amoiroglou, Gregory C. Rogers, Pamela K. Geyerand goat anti-rabbit 800, 1:10,00 LI-COR), and detected using WesternBright Quantum kit (Advansta, K-12042-D10). Purification of sfGFP-BAF variants from Drosophila S2 cells Coding sequences of the sfGFP-BAF and

(9) Failure to Thrive: Impaired BDNF Transport along the Cortical–Striatal Axis in Mouse Q140 Neurons of Huntington’s DiseaseBiology (Basel)January 19, 2023Michael T. Maloney, Wei Wang, Sumana Bhowmick, Ivan Millan, Mridu Kapur, Nicolas Herrera, Everett Frost, Elena Y. Zhang, Scott Song, Melissa Wang, et al.with HRP-conjugated goat anti-rabbit or mouse second antibody and further with WesternBright™ Quantum (K-12042-D10, Advansta, San Jose, CA, USA). Exposed X-ray film was developed in a Kodak developer machine. β-actin

(10) An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitorsGut microbesJanuary 1, 2023Singh R, Rossini V, Stockdale SR, Saiz-Gonzalo G, Hanrahan N, D' Souza T, Clooney A, Draper LA, Hill C, Nally K, et al.for 1 hr, at RT followed by TBST wash and detection with WesternBright Quantum HRP substrate (cat no. K-12042-D10, Advansta) and LAS-3000 Imager (Fujifilm) and processed with ImageJ software (without gel splicing and

(11) DNA methylome and single-cell transcriptome analyses reveal CDA as a potential druggable target for ALK inhibitor–resistant lung cancer therapyExperimental & Molecular MedicineAugust 23, 2022Haejeong Heo et al.IgG (1:5000, 31460, Invitrogen). Immunopositive bands were detected using an ECL kit (K-12045-D50, K-12042-D10, Advansta, CA) and visualized using a Fujifilm LAS-4000 (Tokyo, Japan). siRNA-mediated gene knockdown Small

(12) Exacerbated VEGF up-regulation accompanies diabetes-aggravated hemorrhage in mice after experimental cerebral ischemia and delayed reperfusionNeural regeneration researchJuly 1, 2022Lai AKW, Ng TC, Hung VKL, Tam KC, Cheung CW, Chung SK, Lo ACY.detected using enhanced chemiluminescence reagents (RPN2106, GE Healthcare, Buckinghamshire, UK or K-12042-D10, Advansta, CA, USA) and light-sensitive films (47410 19291, Fujifilm, Tokyo, Japan), quantified using

(13) Expression of Bacillus subtilis ABCF antibiotic resistance factor VmlR is regulated by RNA polymerase pausing, transcription attenuation, translation attenuation and (p)ppGppNucleic Acids ResearchJune 24, 2022Hiraku Takada et al.(Sigma-Aldrich, A0545; 1:10 000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10, Advansta) western blotting substrate and an ImageQuant LAS 4000 (GE Healthcare) imaging system. Functional

(14) HaCaT cells as a model system to study primary cilia in keratinocytesExperimental DermatologyJune 23, 2022Gabriela Blanchard, Christine Pich, Daniel Hohl1. After three TBS‐T washes, membrane was revealed using a WB detection kit WesternBright™ Quantum (K‐12042‐D10; Advansta) according to the manufacturer's instructions. Images were acquired using the Luminescent

(15) Clinically observed deletions in SARS‐CoV‐2 Nsp1 affect its stability and ability to inhibit translationFEBS LettersMay 1, 2022Pravin Kumar, Erin Schexnaydre, Karim Rafie, Tatsuaki Kurata, Ilya Terenin, Vasili Hauryliuk, Lars‐Anders CarlsonSignal™ immunostain (TOYOBO, Osaka, Japan). ECL detection was performed using WesternBright™ Quantum (K‐12042‐D10; Advansta, San Jose, CA, USA) Western blotting substrate and aAmersham™ ImageQuant™ 800 imaging system

(16) Drug screening and genome editing in human pancreatic cancer organoids identifies drug-gene interactions and candidates for off-label treatmentCell GenomicsFebruary 21, 2022Christian K. Hirt et al.Membrane,GE Healthcare,10738328103 BSA Fraction V,Roche,10600015 Western Bright Quantum,Advansta,K-12042-D10 DMSO,Sigma,D8418 BsmBI,NEB,R0580S Fast SYBR Green PCR Master Mix,Thermo Fisher Scientific,4385612 GoScript Reverse Transcription System

(17) TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cellsCell death & diseaseSeptember 23, 2021Woznicki JA, Saini N, Flood P, Rajaram S, Lee CM, Stamou P, Skowyra A, Bustamante-Garrido M, Regazzoni K, Crawford N, et al.secondary antibodies for 1 h at RT. Images were detected with WesternBright Quantum HRP substrate (K-12042-D10, Advansta) on LAS-3000 Imager (Fujifilm) and processed using ImageJ software with brightness/contrast

(18) RqcH and RqcP catalyze processive poly-alanine synthesis in a reconstituted ribosome-associated quality control systemNucleic Acids ResearchAugust 20, 2021Hiraku Takada, Caillan Crowe-McAuliffe, Christine Polte, Zhanna Yu Sidorova, Victoriia Murina, Gemma C Atkinson, Andrey L Konevega, Zoya Ignatova, Daniel N Wilson, Vasili Hauryliuk(Sigma-Aldrich, A0545; 1:10 000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10, Advansta) western blotting substrate and ImageQuant LAS 4000 (GE Healthcare) imaging system. Poly-Ala

(19) Prenatal treatment with rapamycin restores enhanced hippocampal mGluR-LTD and mushroom spine size in a Down's syndrome mouse modelMolecular brainMay 25, 2021Urbano-Gámez JD, Casañas JJ, Benito I, Montesinos ML.HRP-conjugated secondary antibodies were detected with the WesternBright Quantum HRP Substrate (Advansta, Ref. K-12042-D10) and chemiluminiscence measured on a ChemiDoc XRS (BioRad) imager. Immunocytochemistry Hippocampal

(20) Bamboo Shark as a Small Animal Model for Single Domain Antibody ProductionFrontiers in bioengineering and biotechnologyJanuary 1, 2021Wei L, Wang M, Xiang H, Jiang Y, Gong J, Su D, Al Azad MAR, Dong H, Feng L, Wu J, et al.membranes were washed in TBST four times prior to detection with the WesternBright Quantum kit (Advansta, K-12042-D10). For detecting vNARs and VHHs, mouse anti-His tag mAb HRP conjugate (Sino Biological, 105327-MM02T-H)

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WesternBright Quantum HRP Substrate from Advansta

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Specifications/Features

Advansta

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WesternBright Quantum HRP Substrate from Advansta

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